Release of calcitonin gene-related peptide from rat enteric nerves is Ca2+-dependent but is not induced by K+ depolarization.

Hdl Handle:
http://hdl.handle.net/10142/92804
Title:
Release of calcitonin gene-related peptide from rat enteric nerves is Ca2+-dependent but is not induced by K+ depolarization.
Authors:
Belai, A.; Burnstock, G.
Abstract:
The effect of extracellular calcium on the release of calcitonin gene-related peptide (CGRP) induced by electrical field stimulation from enteric nerves of isolated rat ileum was studied; the effect of high potassium, veratridine and caffeine was also examined. Release of endogenous substance P from enteric nerves was also measured for comparison. Electrical field stimulation (10 Hz, 0.3 ms for 2 min) of the ileum preparation caused a significant (P less than 0.001) increase in the release of CGRP and substance P from enteric nerves. The evoked, but not the basal, release of both CGRP and substance P was inhibited in the presence of tetrodotoxin (TTX). The release of CGRP and substance P induced by electrical stimulation was abolished in Ca2+-free medium containing CDTA and also in normal medium containing the calcium channel blocker cadmium chloride (CdCl2), with no change in the level of the basal release of both peptides. However, potassium depolarization (76 and 110 mM) failed to evoke an increase in the release of endogenous CGRP, although it did cause an increase in the release can be induced by mobilization of calcium from intracellular Ca2+ stores. Veratridine, on the other hand, did not cause an increase in CGRP release, although substance P and VIP release was induced by veratridine from the same preparations. The results of the present study have demonstrated that CGRP release from enteric nerves requires the presence of extracellular calcium but, unlike substance P and most other transmitters reported to show calcium-dependent release, potassium depolarization does not induce CGRP release from enteric nerves of rat ileum.(ABSTRACT TRUNCATED AT 250 WORDS)
Citation:
Release of calcitonin gene-related peptide from rat enteric nerves is Ca2+-dependent but is not induced by K+ depolarization. 1988, 23 (2):227-35 Regul. Pept.
Journal:
Regulatory peptides
Issue Date:
Nov-1988
URI:
http://hdl.handle.net/10142/92804
PubMed ID:
2466307
Type:
Article
Language:
en
ISSN:
0167-0115
Appears in Collections:
Department of Life Sciences Collection

Full metadata record

DC FieldValue Language
dc.contributor.authorBelai, A.en
dc.contributor.authorBurnstock, G.en
dc.date.accessioned2010-02-23T22:54:21Z-
dc.date.available2010-02-23T22:54:21Z-
dc.date.issued1988-11-
dc.identifier.citationRelease of calcitonin gene-related peptide from rat enteric nerves is Ca2+-dependent but is not induced by K+ depolarization. 1988, 23 (2):227-35 Regul. Pept.en
dc.identifier.issn0167-0115-
dc.identifier.pmid2466307-
dc.identifier.urihttp://hdl.handle.net/10142/92804-
dc.description.abstractThe effect of extracellular calcium on the release of calcitonin gene-related peptide (CGRP) induced by electrical field stimulation from enteric nerves of isolated rat ileum was studied; the effect of high potassium, veratridine and caffeine was also examined. Release of endogenous substance P from enteric nerves was also measured for comparison. Electrical field stimulation (10 Hz, 0.3 ms for 2 min) of the ileum preparation caused a significant (P less than 0.001) increase in the release of CGRP and substance P from enteric nerves. The evoked, but not the basal, release of both CGRP and substance P was inhibited in the presence of tetrodotoxin (TTX). The release of CGRP and substance P induced by electrical stimulation was abolished in Ca2+-free medium containing CDTA and also in normal medium containing the calcium channel blocker cadmium chloride (CdCl2), with no change in the level of the basal release of both peptides. However, potassium depolarization (76 and 110 mM) failed to evoke an increase in the release of endogenous CGRP, although it did cause an increase in the release can be induced by mobilization of calcium from intracellular Ca2+ stores. Veratridine, on the other hand, did not cause an increase in CGRP release, although substance P and VIP release was induced by veratridine from the same preparations. The results of the present study have demonstrated that CGRP release from enteric nerves requires the presence of extracellular calcium but, unlike substance P and most other transmitters reported to show calcium-dependent release, potassium depolarization does not induce CGRP release from enteric nerves of rat ileum.(ABSTRACT TRUNCATED AT 250 WORDS)en
dc.description.provenanceSubmitted by Abi Belai (a.belai@roehampton.ac.uk) on 2010-02-23T15:02:13Z No. of bitstreams: 0en
dc.description.provenanceApproved for entry into archive by Emily Selvidge(e.selvidge@roehampton.ac.uk) on 2010-02-23T22:54:20Z (GMT) No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2010-02-23T22:54:21Z (GMT). No. of bitstreams: 0 Previous issue date: 1988-11en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCalcitonin Gene-Related Peptide-
dc.subject.meshCalcium-
dc.subject.meshElectric Stimulation-
dc.subject.meshIleum-
dc.subject.meshMale-
dc.subject.meshNeuropeptides-
dc.subject.meshPotassium-
dc.subject.meshRats-
dc.subject.meshRats, Inbred Strains-
dc.subject.meshSubstance P-
dc.titleRelease of calcitonin gene-related peptide from rat enteric nerves is Ca2+-dependent but is not induced by K+ depolarization.en
dc.typeArticleen
dc.identifier.journalRegulatory peptidesen
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