Motor activity and neurotransmitter release in the gastric fundus of streptozotocin-diabetic rats.

Hdl Handle:
http://hdl.handle.net/10142/92789
Title:
Motor activity and neurotransmitter release in the gastric fundus of streptozotocin-diabetic rats.
Authors:
Belai, A.; Lefebvre, R.A.; Burnstock, G.
Abstract:
As deficiencies of the cholinergic and non-adrenergic non-cholinergic innervation of the gastrointestinal tract have been described in diabetic rats, we studied the simultaneous release of, and muscular response to, acetylcholine, vasoactive intestinal polypeptide and adenosine-5'-triphosphate in isolated preparations of gastric fundus from control and 8-week streptozotocin-treated diabetic rats. Muscular responses were measured in longitudinal muscle strips prepared from one half of the gastric fundus and release was studied in the other half. The contractile response to acetylcholine and electrical field stimulation was not different in control and diabetic rats. In the presence of atropine, and when tone was increased with prostaglandin F2 alpha, electrical field stimulation, vasoactive intestinal polypeptide and adenosine-5'-triphosphate induced relaxation with a similar response in control and diabetic rats. The basal release of acetylcholine, vasoactive intestinal polypeptide and adenosine-5'-triphosphate was not significantly different in control and diabetic rats. Electrical field stimulation significantly increased the release of the three substances and this increase was tetrodotoxin-sensitive. While the stimulation-induced increase of acetylcholine and vasoactive intestinal polypeptide was not different in control and in diabetic rats, the stimulation-induced release of adenosine-5'-triphosphate increased 3-fold in diabetic compared to control gastric fundus. Desensitization to alpha,beta-methylene adenosine-5'-triphosphate reduced the relaxant response to adenosine-5'-triphosphate and to electrical field stimulation, suggesting a role of adenosine-5'-triphosphate in non-adrenergic non-cholinergic neurotransmission of rat gastric fundus. The reduction of the non-adrenergic non-cholinergic relaxation by alpha,beta-methylene adenosine-5'-triphosphate was greater in diabetic tissues. This, with the increase in stimulation-induced adenosine-5'-triphosphate release, suggests that the purinergic component of the vagal non-adrenergic non-cholinergic response of the stomach may be increased in diabetics.
Citation:
Motor activity and neurotransmitter release in the gastric fundus of streptozotocin-diabetic rats. 1991, 194 (2-3):225-34 Eur. J. Pharmacol.
Journal:
European journal of pharmacology
Issue Date:
5-Mar-1991
URI:
http://hdl.handle.net/10142/92789
PubMed ID:
1676375
Type:
Article
Language:
en
ISSN:
0014-2999
Appears in Collections:
Department of Life Sciences Collection

Full metadata record

DC FieldValue Language
dc.contributor.authorBelai, A.en
dc.contributor.authorLefebvre, R.A.en
dc.contributor.authorBurnstock, G.en
dc.date.accessioned2010-02-23T15:00:41Z-
dc.date.available2010-02-23T15:00:41Z-
dc.date.issued1991-03-05-
dc.identifier.citationMotor activity and neurotransmitter release in the gastric fundus of streptozotocin-diabetic rats. 1991, 194 (2-3):225-34 Eur. J. Pharmacol.en
dc.identifier.issn0014-2999-
dc.identifier.pmid1676375-
dc.identifier.urihttp://hdl.handle.net/10142/92789-
dc.description.abstractAs deficiencies of the cholinergic and non-adrenergic non-cholinergic innervation of the gastrointestinal tract have been described in diabetic rats, we studied the simultaneous release of, and muscular response to, acetylcholine, vasoactive intestinal polypeptide and adenosine-5'-triphosphate in isolated preparations of gastric fundus from control and 8-week streptozotocin-treated diabetic rats. Muscular responses were measured in longitudinal muscle strips prepared from one half of the gastric fundus and release was studied in the other half. The contractile response to acetylcholine and electrical field stimulation was not different in control and diabetic rats. In the presence of atropine, and when tone was increased with prostaglandin F2 alpha, electrical field stimulation, vasoactive intestinal polypeptide and adenosine-5'-triphosphate induced relaxation with a similar response in control and diabetic rats. The basal release of acetylcholine, vasoactive intestinal polypeptide and adenosine-5'-triphosphate was not significantly different in control and diabetic rats. Electrical field stimulation significantly increased the release of the three substances and this increase was tetrodotoxin-sensitive. While the stimulation-induced increase of acetylcholine and vasoactive intestinal polypeptide was not different in control and in diabetic rats, the stimulation-induced release of adenosine-5'-triphosphate increased 3-fold in diabetic compared to control gastric fundus. Desensitization to alpha,beta-methylene adenosine-5'-triphosphate reduced the relaxant response to adenosine-5'-triphosphate and to electrical field stimulation, suggesting a role of adenosine-5'-triphosphate in non-adrenergic non-cholinergic neurotransmission of rat gastric fundus. The reduction of the non-adrenergic non-cholinergic relaxation by alpha,beta-methylene adenosine-5'-triphosphate was greater in diabetic tissues. This, with the increase in stimulation-induced adenosine-5'-triphosphate release, suggests that the purinergic component of the vagal non-adrenergic non-cholinergic response of the stomach may be increased in diabetics.en
dc.description.provenanceSubmitted by Abi Belai (a.belai@roehampton.ac.uk) on 2010-02-23T14:59:23Z No. of bitstreams: 0en
dc.description.provenanceApproved for entry into archive by Emily Selvidge(e.selvidge@roehampton.ac.uk) on 2010-02-23T15:00:41Z (GMT) No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2010-02-23T15:00:41Z (GMT). No. of bitstreams: 0 Previous issue date: 1991-03-05en
dc.language.isoenen
dc.subject.meshAdenosine Triphosphate-
dc.subject.meshAnimals-
dc.subject.meshCholine-
dc.subject.meshDiabetes Mellitus, Experimental-
dc.subject.meshElectric Stimulation-
dc.subject.meshGastric Fundus-
dc.subject.meshMale-
dc.subject.meshMotor Activity-
dc.subject.meshMuscle Contraction-
dc.subject.meshMuscle, Smooth-
dc.subject.meshNeurotransmitter Agents-
dc.subject.meshRats-
dc.subject.meshRats, Inbred Strains-
dc.subject.meshVasoactive Intestinal Peptide-
dc.titleMotor activity and neurotransmitter release in the gastric fundus of streptozotocin-diabetic rats.en
dc.typeArticleen
dc.identifier.journalEuropean journal of pharmacologyen
All Items in RURR are protected by copyright, with all rights reserved, unless otherwise indicated.