Ultrastructural localization of NADPH-diaphorase and colocalization of nitric oxide synthase in endothelial cells of the rabbit aorta.

Hdl Handle:
http://hdl.handle.net/10142/92773
Title:
Ultrastructural localization of NADPH-diaphorase and colocalization of nitric oxide synthase in endothelial cells of the rabbit aorta.
Authors:
Loesch, A.; Belai, A.; Burnstock, G.
Abstract:
This is the first report on the ultrastructural pattern of distribution of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) in endothelial cells, using the rabbit aorta, and its colocalization with the neuronal isoform (type I) of nitric oxide synthase. About 30% of the endothelial cells showed a positive reaction for NADPH-d compared to about 6% for nitric oxide synthase immunoreactivity. Simultaneous double histochemical-immunocytochemical labelling procedures indicate that all of the cells displaying nitric oxide synthase-positive reactivity also contained NADPH-d; the remainder of NADPH-d-positive endothelial cells were negative for this isoform of nitric oxide synthase. Nitric oxide synthase-immunogold labelling was mostly associated with free ribosomes, while NADPH-d activity was distributed largely in patches in the cytoplasm and in association with the cell membrane.
Citation:
Ultrastructural localization of NADPH-diaphorase and colocalization of nitric oxide synthase in endothelial cells of the rabbit aorta. 1993, 274 (3):539-45 Cell Tissue Res.
Journal:
Cell and tissue research
Issue Date:
Dec-1993
URI:
http://hdl.handle.net/10142/92773
PubMed ID:
7507410
Language:
en
ISSN:
0302-766X
Appears in Collections:
Department of Life Sciences Collection

Full metadata record

DC FieldValue Language
dc.contributor.authorLoesch, A.en
dc.contributor.authorBelai, A.en
dc.contributor.authorBurnstock, G.en
dc.date.accessioned2010-02-23T13:30:32Z-
dc.date.available2010-02-23T13:30:32Z-
dc.date.issued1993-12-
dc.identifier.citationUltrastructural localization of NADPH-diaphorase and colocalization of nitric oxide synthase in endothelial cells of the rabbit aorta. 1993, 274 (3):539-45 Cell Tissue Res.en
dc.identifier.issn0302-766X-
dc.identifier.pmid7507410-
dc.identifier.urihttp://hdl.handle.net/10142/92773-
dc.description.abstractThis is the first report on the ultrastructural pattern of distribution of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) in endothelial cells, using the rabbit aorta, and its colocalization with the neuronal isoform (type I) of nitric oxide synthase. About 30% of the endothelial cells showed a positive reaction for NADPH-d compared to about 6% for nitric oxide synthase immunoreactivity. Simultaneous double histochemical-immunocytochemical labelling procedures indicate that all of the cells displaying nitric oxide synthase-positive reactivity also contained NADPH-d; the remainder of NADPH-d-positive endothelial cells were negative for this isoform of nitric oxide synthase. Nitric oxide synthase-immunogold labelling was mostly associated with free ribosomes, while NADPH-d activity was distributed largely in patches in the cytoplasm and in association with the cell membrane.en
dc.description.provenanceSubmitted by Abi Belai (a.belai@roehampton.ac.uk) on 2010-02-23T13:26:41Z No. of bitstreams: 0en
dc.description.provenanceApproved for entry into archive by Emily Selvidge(e.selvidge@roehampton.ac.uk) on 2010-02-23T13:30:31Z (GMT) No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2010-02-23T13:30:32Z (GMT). No. of bitstreams: 0 Previous issue date: 1993-12en
dc.language.isoenen
dc.subject.meshAmino Acid Oxidoreductases-
dc.subject.meshAnimals-
dc.subject.meshAorta, Thoracic-
dc.subject.meshCells, Cultured-
dc.subject.meshEndothelium, Vascular-
dc.subject.meshImmunoenzyme Techniques-
dc.subject.meshImmunohistochemistry-
dc.subject.meshMale-
dc.subject.meshMicroscopy, Immunoelectron-
dc.subject.meshNADPH Dehydrogenase-
dc.subject.meshNitric Oxide Synthase-
dc.subject.meshRabbits-
dc.titleUltrastructural localization of NADPH-diaphorase and colocalization of nitric oxide synthase in endothelial cells of the rabbit aorta.en
dc.identifier.journalCell and tissue researchen
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