Acrylamide-induced neuropathic changes in rat enteric nerves: similarities with effects of streptozotocin-diabetes.

Hdl Handle:
http://hdl.handle.net/10142/92770
Title:
Acrylamide-induced neuropathic changes in rat enteric nerves: similarities with effects of streptozotocin-diabetes.
Authors:
Belai, A.; Burnstock, G.
Abstract:
The effect of acrylamide intoxication (a widely used model for autonomic neuropathy) on the fluorescence intensity and density of catecholamine- and peptide-containing nerve fibres and tissue content of noradrenaline and the peptides vasoactive intestinal polypeptide, calcitonin gene-related peptide, substance P and neuropeptide Y in the enteric nerves of rat ileum was examined. Histochemical and immunohistochemical techniques were used to localize catecholamine- and peptide-containing nerve fibres. The tissue content of noradrenaline was measured using high-performance liquid chromatography, and an enzyme-linked immunosorbent assay technique was used to determine the tissue content of the peptides investigated. Acrylamide intoxication caused a significant decrease in the density of catecholamine-containing nerve fibres and tissue content of noradrenaline in the myenteric plexus of rat ileum. A decrease in tissue content and immunoreactivity of calcitonin gene-related peptide and an increase in vasoactive intestinal polypeptide was seen in the myenteric plexus of ileum from acrylamide-intoxicated rats. In the submucous plexus, the acrylamide treatment caused a decrease in calcitonin gene-related peptide immunoreactivity and an increase in vasoactive intestinal polypeptide and neuropeptide Y immunoreactivity. There was no change in either tissue content or immunoreactivity of substance P in both myenteric and submucous plexuses of the treated rat ileum. These changes have a striking similarity with those found in the enteric nerves of streptozotocin-diabetic rat ileum, suggesting the possible presence of an underlying common mechanism(s) in the development of neuropathic changes in the autonomic nerves of acrylamide-intoxicated and streptozotocin-diabetic rats.
Citation:
Acrylamide-induced neuropathic changes in rat enteric nerves: similarities with effects of streptozotocin-diabetes. 1996, 58 (1-2):56-62 J. Auton. Nerv. Syst.
Journal:
Journal of the autonomic nervous system
Issue Date:
20-Apr-1996
URI:
http://hdl.handle.net/10142/92770
PubMed ID:
8740660
Language:
en
ISSN:
0165-1838
Appears in Collections:
Department of Life Sciences Collection

Full metadata record

DC FieldValue Language
dc.contributor.authorBelai, A.en
dc.contributor.authorBurnstock, G.en
dc.date.accessioned2010-02-23T13:28:42Z-
dc.date.available2010-02-23T13:28:42Z-
dc.date.issued1996-04-20-
dc.identifier.citationAcrylamide-induced neuropathic changes in rat enteric nerves: similarities with effects of streptozotocin-diabetes. 1996, 58 (1-2):56-62 J. Auton. Nerv. Syst.en
dc.identifier.issn0165-1838-
dc.identifier.pmid8740660-
dc.identifier.urihttp://hdl.handle.net/10142/92770-
dc.description.abstractThe effect of acrylamide intoxication (a widely used model for autonomic neuropathy) on the fluorescence intensity and density of catecholamine- and peptide-containing nerve fibres and tissue content of noradrenaline and the peptides vasoactive intestinal polypeptide, calcitonin gene-related peptide, substance P and neuropeptide Y in the enteric nerves of rat ileum was examined. Histochemical and immunohistochemical techniques were used to localize catecholamine- and peptide-containing nerve fibres. The tissue content of noradrenaline was measured using high-performance liquid chromatography, and an enzyme-linked immunosorbent assay technique was used to determine the tissue content of the peptides investigated. Acrylamide intoxication caused a significant decrease in the density of catecholamine-containing nerve fibres and tissue content of noradrenaline in the myenteric plexus of rat ileum. A decrease in tissue content and immunoreactivity of calcitonin gene-related peptide and an increase in vasoactive intestinal polypeptide was seen in the myenteric plexus of ileum from acrylamide-intoxicated rats. In the submucous plexus, the acrylamide treatment caused a decrease in calcitonin gene-related peptide immunoreactivity and an increase in vasoactive intestinal polypeptide and neuropeptide Y immunoreactivity. There was no change in either tissue content or immunoreactivity of substance P in both myenteric and submucous plexuses of the treated rat ileum. These changes have a striking similarity with those found in the enteric nerves of streptozotocin-diabetic rat ileum, suggesting the possible presence of an underlying common mechanism(s) in the development of neuropathic changes in the autonomic nerves of acrylamide-intoxicated and streptozotocin-diabetic rats.en
dc.description.provenanceSubmitted by Abi Belai (a.belai@roehampton.ac.uk) on 2010-02-23T13:18:04Z No. of bitstreams: 0en
dc.description.provenanceApproved for entry into archive by Emily Selvidge(e.selvidge@roehampton.ac.uk) on 2010-02-23T13:28:42Z (GMT) No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2010-02-23T13:28:42Z (GMT). No. of bitstreams: 0 Previous issue date: 1996-04-20en
dc.language.isoenen
dc.subject.meshAcrylamide-
dc.subject.meshAcrylamides-
dc.subject.meshAnimals-
dc.subject.meshAutonomic Pathways-
dc.subject.meshCalcitonin Gene-Related Peptide-
dc.subject.meshCatecholamines-
dc.subject.meshDiabetes Mellitus, Experimental-
dc.subject.meshDiabetic Neuropathies-
dc.subject.meshIntestines-
dc.subject.meshMale-
dc.subject.meshNerve Fibers-
dc.subject.meshNeuropeptide Y-
dc.subject.meshNorepinephrine-
dc.subject.meshRats-
dc.subject.meshRats, Wistar-
dc.subject.meshSubstance P-
dc.subject.meshVasoactive Intestinal Peptide-
dc.titleAcrylamide-induced neuropathic changes in rat enteric nerves: similarities with effects of streptozotocin-diabetes.en
dc.identifier.journalJournal of the autonomic nervous systemen
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