Plasticity in the myenteric plexus of the rat ileum after long-term sympathectomy.

Hdl Handle:
http://hdl.handle.net/10142/92722
Title:
Plasticity in the myenteric plexus of the rat ileum after long-term sympathectomy.
Authors:
Milner, P.; Lincoln, J.; Belai, A.; Burnstock, G.
Abstract:
To investigate the effect of chronic sympathectomy on the innervation of a tissue with an extensive intrinsic component, 1-week-old rat pups were treated with 50 mg/kg guanethidine for 3 weeks, a treatment shown to produce complete and long-lasting sympathectomy, and the ileum examined. Changes in the levels of noradrenaline, neuropeptide Y, calcitonin gene-related peptide, substance P and vasoactive intestinal polypeptide in the external muscle layers containing the myenteric plexus of the ileum were determined between 6 and 20 weeks of age. After sympathectomy, noradrenaline levels were initially depleted (3% of age-matched controls at 6 weeks, P < 0.001, and 18% of age-matched controls at 12 weeks, P < 0.001), but were not significantly reduced at 20 weeks (67% of age-matched controls). Such increases in noradrenaline content with time after sympathectomy did not occur in the mesenteric vein (levels in 20-week-old sympathectomized rats were 2% of the control values (P < 0.001). In the myenteric plexus, catecholamine fluorescent nerve fibers were seen in the 12-week-old sympathectomized rats, although tyrosine hydroxylase-immunoreactivity was absent. Guanethidine sympathectomy had no effect on the neuropeptide levels in 6-week-old rat ileum but there was a selective increase at 20 weeks; the levels of calcitonin gene-related peptide and substance P were increased (X3, P < 0.001 and X1.6, P < 0.05, respectively) while vasoactive intestinal polypeptide and neuropeptide Y levels were unchanged. Short-term sympathectomy (destruction of sympathetic nerve terminals by acute 6-hydroxydopamine treatment) had no affect on noradrenaline or peptide levels in this tissue.(ABSTRACT TRUNCATED AT 250 WORDS)
Citation:
Plasticity in the myenteric plexus of the rat ileum after long-term sympathectomy. 1995, 13 (5):385-92 Int. J. Dev. Neurosci.
Journal:
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience
Issue Date:
Aug-1995
URI:
http://hdl.handle.net/10142/92722
PubMed ID:
7484209
Language:
en
ISSN:
0736-5748
Appears in Collections:
Department of Life Sciences Collection

Full metadata record

DC FieldValue Language
dc.contributor.authorMilner, P.en
dc.contributor.authorLincoln, J.en
dc.contributor.authorBelai, A.en
dc.contributor.authorBurnstock, G.en
dc.date.accessioned2010-02-23T13:23:07Z-
dc.date.available2010-02-23T13:23:07Z-
dc.date.issued1995-08-
dc.identifier.citationPlasticity in the myenteric plexus of the rat ileum after long-term sympathectomy. 1995, 13 (5):385-92 Int. J. Dev. Neurosci.en
dc.identifier.issn0736-5748-
dc.identifier.pmid7484209-
dc.identifier.urihttp://hdl.handle.net/10142/92722-
dc.description.abstractTo investigate the effect of chronic sympathectomy on the innervation of a tissue with an extensive intrinsic component, 1-week-old rat pups were treated with 50 mg/kg guanethidine for 3 weeks, a treatment shown to produce complete and long-lasting sympathectomy, and the ileum examined. Changes in the levels of noradrenaline, neuropeptide Y, calcitonin gene-related peptide, substance P and vasoactive intestinal polypeptide in the external muscle layers containing the myenteric plexus of the ileum were determined between 6 and 20 weeks of age. After sympathectomy, noradrenaline levels were initially depleted (3% of age-matched controls at 6 weeks, P < 0.001, and 18% of age-matched controls at 12 weeks, P < 0.001), but were not significantly reduced at 20 weeks (67% of age-matched controls). Such increases in noradrenaline content with time after sympathectomy did not occur in the mesenteric vein (levels in 20-week-old sympathectomized rats were 2% of the control values (P < 0.001). In the myenteric plexus, catecholamine fluorescent nerve fibers were seen in the 12-week-old sympathectomized rats, although tyrosine hydroxylase-immunoreactivity was absent. Guanethidine sympathectomy had no effect on the neuropeptide levels in 6-week-old rat ileum but there was a selective increase at 20 weeks; the levels of calcitonin gene-related peptide and substance P were increased (X3, P < 0.001 and X1.6, P < 0.05, respectively) while vasoactive intestinal polypeptide and neuropeptide Y levels were unchanged. Short-term sympathectomy (destruction of sympathetic nerve terminals by acute 6-hydroxydopamine treatment) had no affect on noradrenaline or peptide levels in this tissue.(ABSTRACT TRUNCATED AT 250 WORDS)en
dc.description.provenanceSubmitted by Abi Belai (a.belai@roehampton.ac.uk) on 2010-02-23T13:20:43Z No. of bitstreams: 0en
dc.description.provenanceApproved for entry into archive by Emily Selvidge(e.selvidge@roehampton.ac.uk) on 2010-02-23T13:23:06Z (GMT) No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2010-02-23T13:23:07Z (GMT). No. of bitstreams: 0 Previous issue date: 1995-08en
dc.language.isoenen
dc.subject.meshAdrenergic Fibers-
dc.subject.meshAging-
dc.subject.meshAnimals-
dc.subject.meshAnimals, Newborn-
dc.subject.meshGuanethidine-
dc.subject.meshIleum-
dc.subject.meshImmunohistochemistry-
dc.subject.meshMuscle, Smooth-
dc.subject.meshMyenteric Plexus-
dc.subject.meshNeuronal Plasticity-
dc.subject.meshNeuropeptides-
dc.subject.meshNorepinephrine-
dc.subject.meshOxidopamine-
dc.subject.meshRats-
dc.subject.meshRats, Sprague-Dawley-
dc.subject.meshSympathectomy, Chemical-
dc.subject.meshTyrosine 3-Monooxygenase-
dc.titlePlasticity in the myenteric plexus of the rat ileum after long-term sympathectomy.en
dc.identifier.journalInternational journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscienceen
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